In Vivo Functions of Mouse Neutrophils Derived from HoxB8-Transduced Conditionally Immortalized Myeloid Progenitors

Abstract Although neutrophils play important roles in immunity and inflammation, their analysis is strongly hindered by short-lived terminally differentiated nature. Prior studies reported conditional immortalization of myeloid progenitors using retroviral expression an estrogen-dependent fusion protein the HoxB8 transcription factor. This approach allowed long-term culture mouse (HoxB8 progenitors) estrogen-containing media, followed differentiation toward upon estrogen withdrawal. several reports confirmed vitro functional responsiveness resulting cells neutrophils), little known about capacity to perform vivo neutrophil functions. We have addressed this issue transplantation approach. In vitro–generated showed a neutrophil-like phenotype were able conventional functions, like respiratory burst, chemotaxis, phagocytosis. The i.v. injection into lethally irradiated recipients resulted appearance circulating donor-derived neutrophils. vivo–differentiated migrate inflamed peritoneum phagocytose heat-killed Candida particles. reverse passive Arthus reaction could be induced chimeras but not irradiated, nontransplanted control animals. Repeated also us maintain stable counts for days. Injection arthritogenic K/B×N serum triggered robust arthritis chimeras, mice. Taken together, our results indicate that progenitor–derived are capable performing various providing framework system function.